Immunomodulating properties of Tonsilgon® N

Abstract

Experimental studies on 35 Wistar rats with immunodeficiency caused by cyclophosphamide were conducted to determine the immunomodulating properties of Tonsilgon® N, a fixed herbal combination of Radix Althaeae, Flores Chamomillae, Herba Equiseti, Folia Juglandis, Herba Millefolii, Cortex Quercus and Herba Taraxaci, defining its therapeutic influence. The preparation was administered orally for 10 days, twice daily, in the dosage for adults. The control group included both normal animals that also were given preparation, and the group of animals that underwent any of interferences. Antibody-forming activity (Jerne method), level of natural cellular cytotoxicity (CF- method) and activity of blood cell phagocytosis (latex method) were examined. In statistics a nonparametric test „U“ was used. Administration of cyclophosphamide led to a 2.5-fold antibody decrease and a decrease of natural cytotoxicity by 80% in comparison with control animals. Activity of phagocytosis was inhibited to a lesser degree. Administration of Tonsilgon® N resulted in partial recovery of antibody response in animals with immunodeficiency and in quantity stimulation of antibody producers in normal animals. As regards natural cellular cytotoxicity, Tonsilgon® N renewed the destructive activity of cytotoxic blood cells regarding red cells of chickens (150) in animals with immunodeficiency and increased this activity in normal animals. In group of normal animals the stimulation of phagocytosis was more expressed and defined. It may be assumed that Tonsilgon® N has a broad spectrum of immunomodulating influence either in normal condition or by chemically induced immunodeficiency. Tonsilgon® N seems to modulate both adaptive and innate immunity.