Abstract
High-resolution immunolight microscopy was performed on a series of polypeptide hormone-producing tumors embedded in Spurr plastic. This work revealed that selected antigenic determinants were not destroyed in epoxy and that they could be reexposed by chemical etching and combined with appropriate antisera. It was also clearly demonstrated that the location of the labeled antisera was highly resolved; there was low background staining and the image information was well defined in Spurr sections. Immunocytochemical reactions employing the avidin-biotin conjugated peroxidase method in Spurr correlated with corresponding paraffin-embedded tissues.
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