Abstract
A procedure was developed for rapid detection of Listeria monocytogenes in enrichment broth after 24 h incubation. The technique comprised immunomagnetic separation, by use of paramagnetic labelling with iron particles (size 50 nm) and separation on a high gradient column followed by vital staining and flow cytometry. With this method L. monocytogenes could be separated and detected down to approximately 3×10 4 cells per ml of enrichment broth. Working with different concentrations of L. monocytogenes in the range of 10 4 to 10 7 cells/ml, the bacteria were separated at a recovery rate up to 95%. Using mixed cultures of L. monocytogenes and serologically related species including Lactococcus lactis, the non- Listeria could either be eliminated by the immunomagnetic separation, or be distinguished from L. monocytogenes by the flow cytometric profile.
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