Abstract
Abstract Cell suspension cultures from H.annuus 1805 which release large amounts of extracellular polysaccharides were investigated. It was established that the main amount of polysaccharides was secreted during the logaritmic phase of the growth cycle. Three extracellular polysaccharides of pectic type were isolated from the spent culture medium of H.annuus 1805 alternately by 96% ethanol precipitation and by freezing at −18°C. The polysaccharide isolated by freezing manifested a higher immunostimulating activity. Three GelC fractions were obtained after purification on Sephadex G100. The fraction with the highest molecular weight and the highest content of D-galacturonic acid was the carrier of the biological activity.
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