Abstract
From an alkaline aqueous extract of Phragmites communis, a polysaccharide has been isolated by precipitation with ethanol and further purified by Cellex D and DEAE-Sephadex A-25 column chromatography. The M r was estimated to be 20 000. The specific rotation [α] D 25 (H 2O; c 0.8) was 19°. The component sugars were identified as arabinose, xylose and glucose in a molar ratio of 10:19:94. Anomeric configurations were established by chromium trioxide oxidation and enzymatic reaction. Methylation analysis, periodate oxidation, then reduction and hydrolysis, partial hydrolysis showed that the polysaccharide is a branched arabinoxyloglucan whose backbone is predominantly composed of β(1→4) linked glucopyranosyl residues possessing branching points at position 6. α-Arabinofuranose residues may be present as a part of the non-reducing terminal residues in the side-chains. Another minor α(1→3) linked xylopyranose may be present as a part of the side-chains.
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