Abstract
Two homogeneous, metallic ion-containing pectic polysaccharides with mean M r s of 7600 and 15 000 were isolated from dried aerial parts of Achyrocline satureioides by anion exchange column chromatography on DEAE-Sepharose CL-6B and gel filtration column chromatography on Fractogel TSK HW-50 (S). The structures, as determined by methylation analysis, carboxyl reduction, and partial acid hydrolysis, were shown to be rhamnogalacturonans. Both pectins show a pronounced anticomplementary effect in vitro. The larger carbohydrate AS 4 of higher M r exerts anti-inflammatory activity and a strong enhancement of phagocytosis in vivo.
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