Immunological Properties Evidence by the A. rubens Sea Star Immunoglobulin Kappa Gene

Patricia Otten

doi:10.4172/2157-7013.1000175

Abstract

In 2013-2014, we had discovered a sea star Igkappa gene, showing 2 Ig sites, after star sea immunizations to HRP. By using this gene and introducing it into an Escherichia coli plasmid, we were able to verify that this gene had the property to induce a specific immune response to the enzyme HRP (Horse- radish peroxydase).

Highlights

The general idea that emerged from the experiments, made in our laboratories, was that Echinodermata, as exemplified by sea stars: Asterina gibbosa and Asterias rubens, possessed an immune system, with B sea star lymphocytes, able to mount cellular and humoralspecific responses [1]
A question deserved to be put: did the cloned gene keep the property to induce a specific immune response managed against the used antigen namely HRP (Horse-radish Peroxydase)? It is the object of this work which required, in the first place making an Escherichia coli plasmid and secondarily to perform an enzyme assay by the contribution of its substratum, to see if the « antibody » is bound in the HRP antigen
Our data showed that the E. coli plasmid, obtained from the sea star IgKappa gene, itself from the immunized sea stars to HRP genome, secretes a primitive antibody which is bound in the HRP antigen

Summary

Introduction

The general idea that emerged from the experiments, made in our laboratories, was that Echinodermata, as exemplified by sea stars: Asterina gibbosa and Asterias rubens, possessed an immune system, with B sea star lymphocytes, able to mount cellular and humoralspecific responses [1]. Asterias rubens produced « An antibody » anti HRP, after injections to HRP: it was shown to correlate to kappa genes, in 2011 [2]. In 2013-2014 a sea star Ig Kappa gene to HRP was cloned [3,4]. A question deserved to be put: did the cloned gene keep the property to induce a specific immune response managed against the used antigen namely HRP (Horse-radish Peroxydase)? It is the object of this work which required, in the first place making an Escherichia coli plasmid and secondarily to perform an enzyme assay by the contribution of its substratum, to see if the « antibody » is bound in the HRP antigen A question deserved to be put: did the cloned gene keep the property to induce a specific immune response managed against the used antigen namely HRP (Horse-radish Peroxydase)? It is the object of this work which required, in the first place making an Escherichia coli plasmid and secondarily to perform an enzyme assay by the contribution of its substratum, to see if the « antibody » is bound in the HRP antigen

Materials and Methods

Results

Conclusion