Abstract
Introduction Generation of most immunocompetent cells takes place in bone marrow Bone marrow. As well, bone marrow is a peripheral lymphoid organ where antitumor effector cells and memory cells are present. The aim of the work is to estimate peripheral lymphoid cell subpopulations in bone marrow of lung cancer patients. Materials and methods Study has been done in 68 pts with lung cancer: squamous cell cancer (n = 28), adenocarcinoma (n = 38), other forms (n = 2). In all cases standard diagnostic and staging procedures were performed, as well as morphological (myelogram) and immunological study of bone marrow lymphocyte subpopulations. Multicolor Flow cytomtry was used for study of bone marrow lymphocyte populations. We studied T-cells and its subpopulations, B-cells, NK-cells, perforin-positive cells, and CD27-positive cells. Results Squamous cell lung cancer was characterized by higher content of bone marrow mature T-cells (CD3), and CD8 lymphocytes. More typical for adenocarcinoma was mature B-cell reaction (CD20). Effector (perforin-positive) populations of lymphocytes also were related to histological type of cancer: for adenocarcinoma presence of CD4-positive cytotoxic lymphocytes and CD27-expression on effector cells. Perforin-containing lymphoid cells were in opposite correlation to erythrocaryocytes. Conclusion Subpopulational lymphocyte content of bone marrow is related to histological variant of cancer and erythropoiesis in lung cancer patients.
Highlights
Generation of most immunocompetent cells takes place in bone marrow Bone marrow
Study has been done in 68 pts with lung cancer: squamous cell cancer (n = 28), adenocarcinoma (n = 38), other forms (n = 2)
Multicolor Flow cytomtry was used for study of bone marrow lymphocyte populations
Summary
ИММУНОЛОГИЧЕСКИЕ ПОКАЗАТЕЛИ КОСТНОГО МОЗГА БОЛЬНЫХ НЕМЕЛКОКЛЕТОЧНЫМ РАКОМ ЛЕГКОГО. Н. Костный мозг – место генерации значительной части иммунокомпетентных клеток и в то же время периферический лимфоидный орган, в котором накапливаются эффекторные клетки и клетки памяти противоопухолевого иммунитета. Цель исследования – изучение иммунокомпетентных клеток костного мозга пациентов с немелкоклеточным раком легкого. В ходе исследования плоскоклеточный рак легкого характеризовался более высоким содержанием костномозговых зрелых Т-клеток (CD3) и CD8‐лимфоцитов. Эффекторные (перфоринсодержащие) популяции лимфоцитов костного мозга также имели связь с гистологическим диагнозом рака легкого: для аденокарциномы были более типичны CD4‐положительные цитотоксические лимфоциты и экспрессия на эффекторах CD27. Субпопуляционный состав клеток костного мозга имеет взаимосвязь с гистологическим вариантом рака и эритропоэзом у больных раком легкого. Ключевые слова: рак легкого, костный мозг, Т-клетки, CD8, CD20, CD27, перфорин, эритрокариоциты. Для цитирования: Тупицын Н.Н., Джуманазаров Т.М., Палладина А.Д. Иммунологические показатели костного мозга больных немелкоклеточным раком легкого. Pirogov Russian National Research Medical University, Ministry of Health of Russia; 1 Ostrovitianov St., Moscow 117997, Russia
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