Abstract
Sensitive immunological methods are now available for the detection and quantitation of carcinogen-DNA and protein adducts. Both monoclonal and polyclonal antibodies have been developed against DNA modified by benzo(a)pyrene diol epoxide-I (BPDE-I). These antisera recognize a number of structurally related diol epoxide-DNA adducts, and have been utilized in enzyme-linked immunosorbent assays (ELISAs) to determine adduct levels in DNA isolated from humans with several different environmental and occupational exposures to polycyclic aromatic hydrocarbons (PAHs). Populations studied include cigarette smokers, foundry workers, coke oven workers, and coal tar treated psoriasis patients. Since these populations are exposed to complex mixtures of PAHs, multiple adducts will be formed and determined by the ELISA. In addition, the antibodies can be used in immunohistochemical studies to investigate localization of adducts to specific cell and tissue types.
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