Abstract
The proliferative effects of the mitogens phytohaemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), and staphylococcal protein A (SpA) were investigated using two different methods which enable immunological marker analysis of proliferating cells: either surface marker labelling followed by BrdU incorporation or screening of metaphases after surface marker labelling. Therefore peripheral blood mononuclear cells from six healthy volunteers were stimulated with these four mitogens. Both PHA and Con A gave rise to more CD8+ than CD4+ proliferating cells. PHA, but not Con A, induced B-cell proliferation as well. PWM mainly caused T-cell proliferation. SpA also appeared to be a potent T-cell mitogen in addition to its capacity to induce B-cell proliferation. However, in contrast to the other mitogens SpA predominantly stimulated CD4+ cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
