Abstract
A linear UV dose response is demonstrated using an ELISA assay that measures thymine dimers formed in the genomic DNA extracted from the mixed species of microorganisms filtered from UV treated pre-disinfection drinking water.
Highlights
The purpose of a drinking water UV disinfection system is to ensure that all water passing through it receives at least the minimum required dose of UV light in order to achieve the required levels of inactivation of target microorganisms found in the inflow stream
Optimisation of the mouse monoclonal antithymine dimer H3 clone primary antibody (H3Ab) for detection of dimers in naked E. coli gDNA irradiated with 500 mJ cm−2 is shown in Fig. S.3 and S.4 in the Electronic supplementary information (ESI).† All signals appeared to be specific to the enzyme-linked reporter on the secondary antibody, as very low signal levels were seen in controls
Due to the lack of a measurable residual, verification of dose delivery by UV disinfection systems in operation relies on indirect methods
Summary
The purpose of a drinking water UV disinfection system is to ensure that all water passing through it receives at least the minimum required dose of UV light in order to achieve the required levels of inactivation of target microorganisms found in the inflow stream. The intensity of UV light in a flow-through system decreases according to the square of the distance from the mercury arc lamp. All municipal flow-through UV disinfection systems have at least one static UV sensor that measures the internal UV light intensity at a single point. Because UV disinfection systems deliver a UV dose distribution, there is no direct correlation between the single-point UV intensity. Sci.: Water Res. Technol., 2021, 7, 2010–2020
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