Abstract
Antisera were prepared against homogeneous serine hydroxymethyltransferase (SHMT) of an obligate methylotroph, Hyphomicrobium methylovorum GM2. Cell-free extracts of methylotrophic and non-methylotrophic microorganisms, rat and rabbit liver, which showed SHMT activities, were tested for immunological corss-reactivity with the anti-H. methylovorum GM2-SHMT. The extracts of only six methylotrophic strains showed reactivities These bacterial strains were all found to belong to the genus Hyphomicrobium on the basis of electron microscopy and C1-compound utilization. The cross-reaction test using the anti-H. methylovorum GM2-SHMT was also applied to various Hyphomicrobium strains. With the exception of H. neptunium, which has previously been recommended to be transferred to another genus, cell-free extracts of all the strains formed precipitin lines; some of them fused and the other spurred with each other. Thus, it was concluded that SHMT enzymes of methylotrophic Hyphomicrobium strains are immunologically related closely to each other and that the SHMT of H. methylovorum is an antigen specific for the genus Hyphomicrobium.
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