Abstract
Stage-specific cell surface antigens expressed during mouse preimplantation development and detected by a rabbit antiserum prepared against mouse blastocysts (A-BL 2) have been characterized by serological and biochemical techniques. Immunofluorescence, immunoradiolabeling, and complement-mediated cytotoxicity assays reveal the expression of A-BL 2 surface antigens beginning at the 4-cell stage and reaching a maximum at the 8-cell to morula stages. At earlier times in development A-BL 2 antigens are not detectable, and there is a decline in expression at the blastocyst stage. No antibody reactivity is detected against adult mouse tissues or teratocarcinoma cell lines. The presence of A-BL 2 antibodies during in vitro embryo culture interferes with normal development. Treatment of embryos with β- N-acetylglucosaminidase, but not other glycosidases, proteases, or lipases, results in a quantitative decrease in the binding of A-BL 2 antibodies to surface antigens. Immunoprecipitation and electrophoretic analyses of A-BL 2 antigens demonstrate specific antibody activity against a pair of embryonic glycoproteins of 65,000 to 70,000 daltons which can be metabolically labeled with 35S-methionine and 3H-glucosamine. Tunicamycin treatment alters the form of the A-BL 2 immunoprecipitate to a single 60,000-dalton protein.
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