Abstract
The inoculation of the European Alnus glutinosa (L) Gaertn host plant by a pure culture of an actinomycete isolated from the North American Comptonia peregrina (L) Coult root nodule was successful. Fluorescein-labeled antibodies, specific against the actinomycetal isolate of the C peregrina root nodule, demonstrated the identity of this actinomycete in the resulting Alnus root nodules The nodulation process of this abnormal host-endophyte system was studied by light and electron microscopy The actinomycetal intruder was branched, and septate hyphae produced septate vesicles These endophyte vesicles were spherical like the Alnus-type instead of club shaped like the Comptonia-type The endophyte hyphae and vesicles were always encapsulated in a polysaccharide material surrounded by a host plasma-membrane envelope The Comptonia isolate, which forms spores in pure culture, did not sporulate inside the Alnus root nodule. In this abnormal host-endophyte system, appearance of the root nodules was delayed for 1-2 ...
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