Immunological and molecular techniques used for determination of serotypes in Pasteurellaceae

Abstract

Abstract Serotyping has been used to categorize most pathogenic members of Pasteurellaceae of human or animal importance. In particular, serotyping of isolates from vaccine breaks is of primary importance to ensure that vaccines used are covering outbreak isolates in the region. Traditional serotyping methods are available for Pasteurella multocida, Actinobacillus pleuropneumoniae, Glaesserella parasuis (older name Haemophilus parasuis), Mannheimia haemolytica, Avibacterium paragallinarum, Haemophilus influenzae, and Aggregatibacter actinomycetemcomitans. PCR-based methods to predict the serotypes have been developed for all agents with the exception of Avibacterium paragallinarum. For several of the species, work is in progress to predict serotypes from whole genomic sequences. The combination of population structures determined by whole genomic sequencing and the prediction of serotypes provides a strong synergy in epidemiological investigations of the species of Pasteurellaceae involved in disease in humans and animals.