Immunological and Molecular Diagnosis of Cytomegalovirus Infection between Aborted &amp; Pregnant Women in Babylon City

Haider Turky Mousa Al-Mousawi,Qabas Neamah Al-Hajjar

doi:10.21123/bsj.2021.18.2(suppl.).1086

Abstract

Human cytomegalovirus (CMV) is the globally highly prevalent herpesvirus worldwide. CMV infects populations of all ages according to the Center for Disease Control and Prevention (CDC) and World Health Organization (WHO). CMV infections remain the most common viral complication potentially multiple in humans and are a major cause of congenital normality in women, which is why they are critical for diagnosis in several times when it happens during pregnancy. Pregnant women with CMV infection can be in charge of abortion or congenital expandaedby. This study involves the collection a total of (90) samples taken from each aborted and pregnant woman (70 with abortion cases and 20 of pregnant without history of abortion as control subjects) referring to Babylon teaching hospital for Maternity and Children, covering a period from (October 2018 to March 2019) to investigate the occurrence of Cytomegalovirus (CMV) in Babylon city. Patients and controls were evaluated for IgG, IgM antibodies and anti-HCMV IgG, IgM for (90) subjects were controlled in this study using the Enzyme Immunoassay Test Kit and read by enzyme - linked immunosorbent analyze (ELISA). In addition the polymerase chain reaction (PCR) DNA detection for CMV are based on the amplification of pathogen genomes in a particular region using different primers. The Chi-square test was used to analyze the data. The results show among 90 samples, women were evaluated for CMV infection, the seroprevalence titer was significantly higher at P <0.05 in seropositive cases ranging from 62(89%) toward positive CMV IgG, while the 65 (93%) of patients were positive CMV IgM from (70) women with abortions. By contrast, the results obtained from the controls were 9 (45%) subjects seropositive for IgG and all of them were seronegative with IgM. The anti – HCMV IgG finding showed high positivity that represents the furthermost of CMV infections among females through ages ranging between 20-29 years. Furthermore, the outcomes of molecular detection showed that a small number of samples 13 (19%) were HCMV DNA detectable in aborted women less than in pregnant women 3 (15%).

Highlights

Human cytomegalovirus (HCMV) as an opportunistic pathogen is known as the omnipres ent herpes virus (Human Herpes Virus 5) from the characteristic cytomegalic appearance of intra-nuclear inclusions in most cell types and organs, with the highest morbidity and mortality rates compared with other herpes virus, with genomes consisting of mono partite, linear, double-stranded DNA and is roughly 235 kbp size capable of a wide spectrum of disease in humans [1]
Materials and Methods: Clinical Samples Blood and plasma samples have been obtained as women (70 patients travail from spontaneous abortion and 20 pregnant women as a comparable group) during the period from October 2018 to March 2019 at consultant clinic for infertile women in Babylon (Babil Teaching Hospital for Maternity and children)
During pregnancy, reactivated infections or maternal primary infections can lead to congenital HCMV infections, but maternal re-infection with different CMV strains can rarely lead to congenital symptom infections

Summary

Introduction

Human cytomegalovirus (HCMV) as an opportunistic pathogen is known as the omnipres ent herpes virus (Human Herpes Virus 5) from the characteristic cytomegalic appearance of intra-nuclear inclusions in most cell types and organs, with the highest morbidity and mortality rates compared with other herpes virus, with genomes consisting of mono partite , linear, double-stranded DNA and is roughly 235 kbp size capable of a wide spectrum of disease in humans [1]. The recurrence of pregnancy waste due to maternal infections spread in the uterus at various gestational stages may be caused by a wide range of pathogens, including the TORCH complex (Toxoplasma gondii, Rubella virus, Cytomegalovirus and Herpes simplex virus) [3, 4]. These entire infective agents induce a shift of responding of the immunity through pregnancy from Th2 to Th1 with apoptosis which can be experiential clinical as abortion progression [5]. This objective was carried out by the following objectives: Microbial agents detection of such as (IgM, IgG) Cytomegalovirus and by ELISA Technique and Genetic detection of Cytomegalovirus DNA in collected samples

Objectives

Methods

Results