Abstract
Publisher Summary This chapter discusses a chromatin immunoprecipitation assay that helps probe the organization of the protein–DNA complexes that exist within a cell. For small organisms such as the budding yeast, this provides an approach to the molecular cytology of chromosomes that are too small to be visualized microscopically. With the budding yeast, the availability of a wide array of molecular genetic tools as well as a complete genome sequence makes effective application of this technique straightforward. Even with organisms with more complex genomes and less tractable molecular genetics, the technique has been and can be applied to great advantage. Polymerase chain reaction (PCR) analysis of precipitated DNA has extended the limit of sensitivity of the technique, thereby making a small genome less of a constraint. Careful attention to biochemical and immunological controls can obviate the need for extensive genetic controls. The procedure has been used for examining the effects of mutations on the structure and organization of particular chromosomal structures.
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