Abstract

Rabbit antisera were prepared against a synthetic peptide corresponding to the carboxyterminal amino acid sequences of the transforming protein p110 gag-myc of avian oncovirus MC29. Analysis of immunoprecipitates formed with these sera (anti- mycC) demonstrated that the gag-myc hybrid proteins encoded by the avian leukemia viruses MC29, CMII, and OK10 were recognized by the anti-peptide sera, but not the gag or pot precursor proteins of helper viruses or the MH2-encoded p100 gag-mil protein. In cells transformed by OK10 or MH2, putative v- myc proteins of 60K (OK10) and 59/61K (MH2) were also precipitated by anti- mycC. In addition, the anti-peptide sera reacted specifically with the gag-myc proteins encoded by three partially transformation-defective mutants of MC29, td10A, td10C, and td10H, and by MC29 variant HBI.

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