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Abstract
Sperm motility inhibiting factor (SMIF), isolated from boar seminal plasma, injected i.p. into mice at a dose of 100 μg, inhibited the humoral immune response against T-cell dependent antigen (sheep red blood cells) in Cunningham tests. SMIF given in doses of 12–100 μg potentiated the proliferation of murine splenocytes stimulated by Concanavalin A but there was no dose-dependent effect. SMIF also affected the cell-mediated immune response against histocompatibility antigens in skin graft tests in mice. Immunofluorescence analysis showed that SMIF exists on the membranes of boar, bull, ram and human spermatozoa.
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