Immunologic, chemical and isotopic identification of C-14-labeled bacterial polysaccharide in animal tissues.

Abstract

SummaryPolysaccharide isolated from encapsulated Klebsiella pneumoniae, type B, has been found to consist of glucose, glucuronic acid and rhamnose. C14 was incorporated biosynthetically into these 3 components from acetate- 1-C14 added to the media. Following injection of the C14-labeled polysaccharide into animals, up to 80% of the labeled material has been recovered from animal tissue by a combination of enzymatic and extractive procedures. Much of the C14-la-beled material in tissue retained its original haptenic properties and relative C14 content of the constituent monosaccharides.