Abstract
Background L-glutamate protects hearts against myocardial stunning and infarction while glutamate receptors antagonists abolish the protection. We report immunolocalization (IL) of glutamate Receptor 5 (mGluR5) in isolated rabbit normoxic (rode-shaped) or ischemic contracture (round-shape) cardiomyocytes and in H9C2 cells. Methods Collagenase-dispersed rabbit heart cells, attached and fixed on glass slides, underwent blocking of the non-specific binding, labeling with mGluR5 antibody, biotinylated secondary antibody and ultra-Vidin Texas Red as a fluorescent probe, and examined under fluorescent microscopy. H9C2 cells were cultured on cover slips and treated as above and examined under fluorescent and confocal microscopy. Results Positive IL of mGluR5were evenly distributed on cell membranes of normoxic rabbit cardiomyocytes but clustered and internalized in cells underwent contracture. IL of mGluR5 in cultured H9C2 cells was positive on the surface membranes and on the mitochondria around the myofibrils. Conclusions mGluR5 are localized on membranes of rode-shaped cardiomyocytes while undergo clustering and internalization during contracture. mGluR5 found in H9C2 on cells membranes and mitochondria. Further studies are warranted to elucidate the cellular and molecular signaling mechanisms of mGluR5 activation (or blockade) and internalization in response to oxidative stress.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
