Abstract

Localization of lipoxygenase (LOX) in the microspore of <i>Gagea lutea</i> (L.) Ker.-Gaw. was investigated with the immunogold labelling method. The enzyme was found in the cytoplasm, nucleus and sporoderm. The most intensive reaction was observed in the cytoplasm, where the immunogold particles were sometimes grouped into clusters of several or more and showed the highest density. The smallest amount of particles occured in the sporoderm. The role of lipoxygenase in the microspore is discussed.

Highlights

  • Roœliny Gagea lutea (L.) Ker.-Gaw. (Liliaceae) zbierano z naturalnych stanowisk w okolicach Nowego Nartu w styczniu 2003

  • The enzyme was found in the cytoplasm, nucleus and sporoderm

  • The most intensive reaction was observed in the cytoplasm, where the immunogold particles were sometimes grouped into clusters of several or more and showed the highest density

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Summary

Introduction

Roœliny Gagea lutea (L.) Ker.-Gaw. (Liliaceae) zbierano z naturalnych stanowisk w okolicach Nowego Nartu w styczniu 2003. Immunolokalizacja lipoksygenazy w mikrosporze Gagea lutea (L.) Ker.-Gaw. EWA SZCZUKA1, BOENA PAWLIKOWSKA-PAWLÊGA2, EWA SKÓRZYÑSKA-POLIT3, JOLANTA SOBIESKA4, JAROS£AW PAWELEC2, TOMASZ PIERSIAK2 Immunolocalization of lipoxygenase in the microspore of Gagea lutea (L.) Ker.-Gaw. Summary Localization of lipoxygenase (LOX) in the microspore of Gagea lutea (L.) Ker.-Gaw. was investigated with the immunogold labelling method. W prezentowanej pracy przedstawiamy rozmieszczenie lipoksygenazy w mikrosporze z3oci ¿ó3tej (Gagea lutea) przy u¿yciu metody immunoz3otowej.

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