Abstract
Summary: Fibroblast growth factors (FGF) regulate cell proliferation, migration, differentiation and angiogenesis during morphogenesis in many different tissues. Recent evidence indicates that exogenous FGF‐2 stimulates mesenchymal condensation in cultured rat metanephroi, a crucial epithelial‐mesenchymal induction event in the developing nephron. the aim of the present investigation was to determine the in vivo distribution of FGF‐1 and FGF‐2 in developing rat metanephroi at embryonic days 14, 15, 16, 18 and 20. Avidin‐biotin enhanced indirect immunohistochemistry was used to demonstrate that both FGF‐1 and FGF‐2 were co‐localized in metanephroi at all ages studied. High levels of FGF‐1 and FGF‐2 were present in ureteric bud branches and in developing distal tubules. Fibroblast growth factor‐1 and FGF‐2 were colocalized in developing nephron elements, from vesicles to S‐shaped bodies, and in the mesangium of capillary loop and maturing stage glomeruli. Both growth factors were present in the mesenchyme of the nephrogenic zone and in the interstitium of the developing cortex. However, immunostaining for FGF was not evident in mesenchymal condensates, endothelial cells, medullary interstitial cells, or in the thin undifferentiated epithelium of the immature loop of Henle. These findings indicate that the expression of both FGF‐1 and FGF‐2 is tightly regulated in the embryonic kidney and suggest a role for these molecules in kidney development.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.