Abstract
To investigate the proliferative potencies of thetwo types of synovial lining cells (types A and B), we usedimmunohistochemical techniques under light and electronmicroscopy to survey the complete process of arthritis in a ratmodel system. Complete Freund’s adjuvant (0.1ml) containing5 mg of heat-inactivated Mycobacterium butyricum wasadministered intradermally into the right hind paws of 25 maleSprague-Dawley rats. Five animals were killed at weeks 1, 2, 3,and 4 after the immunological challenge, respectively, andthe infrapatellar adipose synovium was removed from eachanimal. Using proliferative cell nuclear antigen (PCNA) as acell proliferation marker, we quantified the locations ofproliferative cells under light microscopy and then identifiedthe cell type with immunoelectron microscopy. The number ofPCNA-positive cells was high in the deep layer at week 2, andmost of them were determined to be B cells. At week 3, thenumber of PCNA-positive cells increased in the superficiallayer, and most were identified as A cells. Our results supportthe idea that cells of the synovial lining proliferate in situduring the course of arthritis.
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