Immunohistochemical Study of Equine Endometrial Extracellular Matrix during the Oestrous Cycle

Abstract

This study was designed to identify the distribution of type IV collagen, laminin, and fibronectin with an avidin-biotin method in sections of equine endometrial samples, fixed in Bouin's solution and embedded in paraffin wax. Thirty endometrial biopsies were collected at three different stages of the oestrous cycle. The basement membrane of luminal epithelium reacted positively with antibody against type IV collagen. Both type IV collagen and laminin were found in the basement membranes of endometrial glands, and fibronectin occurred diffusely in the interstitial tissue. Blood vessels expressed all of the extracellular matrix components studied. No differences in the distribution of extracellular matrix components were found at the different stages of sampling.