Abstract
Obectives:To evaluate the expression level, localization and distribution of 14-3-30 ans proteins in human ovarian epithelial carcinoma cells. Methods: Epithelial carcinoma, benign epithelial tumor and normal ovaran tissues were evaluated by immunohistochemistry. For this study, mucinous adenoma (n=10), serous adenoma (n=10), mucinous adenocarcinoma (n=10) and serous adenocarcinoma (n=10) of ovary and nomal ovarian tissue (n=10) were analyzed. Goat anti 14-3-30 and palyclonal antibody and rabbit anti-14-3-3 polyclonal antibody were used as primary antibodies. Results: The 14-3-30 protein expression in the glandular epithelium was not statistically different among normal ovary, cystadenoma and cystadenocarcinoma. The 14-3-3 protein were immunohistochemically demonstrated in the nudei and more preferably in the cytoplasm of adenocarainoma epithelial cells. Conclusion: The expression of 14-3-3 protein increased significantly in ovarian adenocarcinoma compared with adenoma and normal ovary. It is suggested that 14-3-3 protein expression may play an important role in abnormal growth of ovarian epithelial tumor cell.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
