Abstract
Vitellogenin (VG) was localized in the cytoplasm of hepatocytes in freeze-dried, plastic-embedded and Bouin's-fixed, paraffin-embedded sections of channel catfish liver. The location of VG was demonstrated by using a peroxidase–antiperoxidase immunohistochemical method. Positive immunostaining was obtained only in naturally vitellogenic females and in fish that had been injected with 2 mg/100 g body weight 17β-estradiol. Vitellogenin reaction product was observed in most but not all of the hepatocytes from estradiol-induced and naturally vitellogenic fish. Glycogen content was reduced in hepatocytes that showed positive VG immunostaining. Nuclear size was larger in hepatocytes from vitellogenic fish compared with nonvitellogenic fish.
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