Abstract
Immunohistochemical localization of chondroitin sulphate and dermatan sulphate proteoglycans (PGs) was observed in 70 tumour tissues, using monoclonal antibodies 9A-2 and 3B-3 raised against core molecules obtained from chondroitin sulphate PG by chondroitinase ABC-treatment. They recognize a stub of delta Di-4S and delta Di-6S binding to core protein via a linkage tetrasaccharide, respectively. The antibody 6B6 raised against dermatan sulphate PG obtained from an ovarian fibroma capsule in our laboratory was also used. The interstitial fibrous elements, so-called 'specific stroma' within the cancer cell nests contained chondroitin 4-sulphate PG as revealed with 9A-2, whereas the surrounding connective tissue and the preexisting fibrous connective tissue involved in the tumour growth consisted of dermatan sulphate PG with a considerable amount of chondroitin 4-sulphate PG. Chondroitin 6-sulphate PG as revealed with 3B-3 was located in the connective tissue proliferating from blood vessels and muscle tissue in association with the invasive growth of tumour cells. Chondroitin 6-sulphate PG was also observed in the basement membrane components of some tumours. In non-epithelial tumours (fibrogenic, chondrogenic, osteogenic and neurogenic tumours), chondroitin 4-sulphate was in fibrous portions. When collagenization and hyalinization progressed, dermatan sulphate PG was observed to increase in quantity.
Highlights
Monoclonal antibodies used in the present study were: 9A-2 and 3B-3 (Couchman et al, 1 984a, b) and the gifts of Seikagaku Kogyo Company Ltd., Tokyo
The results suggest that antibody 6B6 reacts with small dermatan sulphate PG-IT, which was designated by Rosenberg et al (1985)
The epitope of the antibody 9A-2 is a stub of ADi-4S unit remaining on a core molecule of chondroitin sulphate PG and dermatan sulphate PG after chondroitinase ABCtreatment
Summary
Monoclonal antibodies used in the present study were: 9A-2 (against a stub of ADi-4S unit binding to core protein obtained from chondroitin sulphate PG by chondroitinase ABC) and 3B-3 (against a stub of ADi-6S unit binding to core protein obtained from chondroitin sulphate PG by chondroitinase ABC) (Couchman et al, 1 984a, b) and the gifts of Seikagaku Kogyo Company Ltd., Tokyo. Antibodies against dermatan sulphate PG, which was purified from the capsular tissue of a human ovarian fibroma (Sobue et al, 1987), were produced by the conventional hybridoma technique in our laboratory. One of the monoclonal antibodies, IgGI, designated 6B6, was found to react with the intact molecule of the antigen (dermatan sulphate PG) and the chondroitinase AC-treated core molecule on Western blotted nitrocellulose membrane. After excision, tissue slices 5 mm thick were cut from the excised tissues and fixed in glacial acetic acid
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