IMMUNOHISTOCHEMICAL INVESTIGATIONS OF P16 INK 4A EXPRESSION IN CARCINOMAS AND HIGH GRADE CERVICAL LESIONS

Abstract

SUMMARY: During the years the role of viruses as a cause of oncological illness was known. One of them is cervical carcinomas, related to Human papilloma viruses (HPV’s). There are about 450000 new cases of cervical cancer worldwide each year, and 250000 deaths of this diagnosis. By the diagnosis cervical cancer get there with two procedures: after mass scrinning or clinical suspicious for ill. By the availability apparent tumor diagnosis is put with biopsy. By the discreet lesions require utilization modern diagnostic methods. One of them is immunohistochemical method. In situ hybridization (ISH) assays for high-risk human papillomavirus (HR-HPV) and immunohistochemical (IHC) assays for surrogate markers such as p16 INK 4a can be useful in detecting HR-HPV in cervical dysplasia. The aim of the following study was to find difference the level for expression of the tumor specific gene p16 INK 4a in reactive, dysplastic and invasive changes in biopsy specimens from uterine cervix. In the lesions of the uterine cervix, overexpression of p16 INK 4a is induced by HPV and is associated with the process of cancerogenesis in the epithelium. Almost 100% of the high grade lesions and the invasive carcinomas demonstrated high level of expression of p16 INK 4a while non displastic epithelium is always negative.