Immunohistochemical expression of PTEN (phosphatase and tension homolog) in endometrial carcinoma

Abstract

Background and objective: Different molecular alterations have been described in the pathogenesis of endometrial carcinoma, among themis mutation of the PTEN (phosphatase and tension homolog), a tumor suppressor gene. This study aimed to detect PTEN immunoexpression in endometrial carcinoma and to asses the association between PTEN immunoexpression with the clinicopathological parameters. Methods: This cross-sectional retrospective study was carried out between January 2016-December 2021 in Erbil city. A total of 112 formalin fixed, paraffin embedded, archival tissue blocks of total abdominal hysterectomy samples were collected in Erbilcity. The clinicopathological characteristics of the tumors were revised, and the specimens were analyzed immunohistochemically using monoclonal PTEN antibody clone 6H2.1 (Dako. Results: Seventy- seven cases (68.8%) were labeled as negative for PTEN immunoexpression. While 35 cases (31.2%) were labeled as positive for PTEN immunoexpression. PTEN immunoexpression was significantly associated with the tumor histological types (P = 0.001), while no significant association was found between PTEN immunoexpression and other clinicopathological parameters. Conclusion: Loss of PTEN immunoexpression frequently seen in endometrioid type of endometrial carcinoma than other types of endometrial carcinoma and is significantly associated with the histological types of endometrial carcinoma.