Immunohistochemical Expression of Cyclin D1 and Ki-67 in Primary and Metastatic Oral Squamous Cell Carcinoma.

Maryam Nazar,Shoaib Naiyer Hashmi,Muhammad Khurram Mahmood,Iram Naz

doi:10.31557/apjcp.2020.21.1.37

Abstract

Objective:The aim of current study was to investigate the expression of Cyclin D1 and Ki-67 in primary and metastatic oral squamous cell carcinoma (OSCC) and their different histological grades. Methods:Paraffin embedded 30 oral squamous cell carcinoma (15 each of primary and cervical lymph node metastatic OSCC) were included in the study. Cyclin D1 and Ki 67 expressions were evaluated by immunohistochemistry and compared in primary and lymph node metastasis of OSCC and their histological grades. The data was analyzed using SPSS software. Results:The mean age of patients with primary OSCC was 53.47 ±16.67 years and 61.47 ±11.94 years in patients with metastasis. Males were comparatively affected more than females with tongue as the most common site involved in both primary and metastatic tumours. The mean size of primary and metastatic tumour biopsies were 1.16 mm and 3.93 mm respectively. Comparison of the expression of Cyclin D1 in these primary and metastatic OSCC revealed a statistically significant difference (p = 0.003) whereas it was insignificant for Ki-67 (p = 0.715). Conclusion: Cyclin D1 can be a useful marker in predicting aggressive or metastatic behaviour of OSCC on premier biopsies.

Highlights

Oral cancers are claimed to account 2-4 % of all cancers worldwide and amongst all Oral Squamous Cell Carcinoma (OSCC) is the most frequently occurring
The aim of current study was to investigate the expression of Cyclin D1 and Ki-67 in primary and metastatic oral squamous cell carcinoma (OSCC) and their different histological grades
Cyclin D1 and Ki 67 expressions were evaluated by immunohistochemistry and compared in primary and lymph node metastasis of OSCC and their histological grades

Summary

Introduction

Oral cancers are claimed to account 2-4 % of all cancers worldwide and amongst all Oral Squamous Cell Carcinoma (OSCC) is the most frequently occurring. It constitutes over 90% of malignancies found in the mouth and oropharynx; considered malignant due to its infiltrative capacity and early tendency to metastasis (Yakob, 2014; Acero-Mondragón et al, 2016). Cyclin D1 is a vital proto oncogene that has a major role in cell cycle regulations through G1 to S phase progression It is important for the development and progression of several cancer types, including oral epithelial cancer. Over expression of CyclinD1 is reported to be associated with recurrence and shortened overall survival in curable cases of squamous cell carcinoma of head and neck (SCCHN) (Shiang-Fu et al, 2012)

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Results

Conclusion