Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma.

Maria Verônica De Souza,Marianna Barros De Souza Lima,Mariana Brettas Silva,Rosy Iara Maciel De Azambuja Ribeiro,Hélio Batista Dos Santos,Ralph Gruppi Thomé,Gabriela Francine Martins Lopes,Júlio Crepaldi,José De Oliveira Pinto

doi:10.1155/2015/893485

Maria Verônica De Souza, Marianna Barros De Souza Lima + Show 7 more

Open Access

https://doi.org/10.1155/2015/893485

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Abstract

This study evaluated the immunohistochemical expression of type I (COL I) and III (COL III) collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma (LP-PRP). Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control (CG). Three samples were obtained: after wound induction (T0), 14 days (T1) of healing process, and after complete closure of the skin (T2). The normal skin (T0) showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important (p < 0.05) increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.

Highlights

Large animals are considered excellent models for the study of wounds, given that they provide the proper comparison of the many physiological and immunological aspects involved in wound healing [1]
Cytokines, and extracellular matrix molecules at the wound site interact with different systemic factors such as platelets, the coagulation cascade, and humoral cell components, which together enable the healing of wounds [2]
The procedures were conducted according to the Rules of Conduct for the Use of Animals in Teaching, Research and Extension of the Department of Veterinary (DVT/Universidade Federal de Vicosa (UFV)), the Medical Veterinary Professional Ethics Code, the Ethical Principles for Animal Research established by the Brazilian College for Animal Experimentation (COBEA), and the current Brazilian Legislation

Summary

Introduction

Large animals are considered excellent models for the study of wounds, given that they provide the proper comparison of the many physiological and immunological aspects involved in wound healing [1]. The most appropriate form of healing a cutaneous wound is by first intention [3, 4], but this procedure is limited to wounds located in anatomical regions that allow for the excision and adaptation of its edges [3]. On the other hand, which involves two independent processes—contraction and reepithelialization [5]—is often the only possibility to close wide and deep wounds. The process is slow and may result in the formation of exuberant granulation tissue. In this sense, new therapies aiming to reduce the maximum healing period of cutaneous

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