IMMUNOHISTOCHEMICAL EXPRESSION OF AMELOGENIN AND DENTIN SIALOFOSFOPROTEIN IN TEETH WITH AMELOGENESIS IMPERFECTA, DENTINOGENESIS IMPERFECTA AND REGIONAL ODONTODYSPLASIA

Abstract

Objective To compare the immunohistochemical expression of amelogenin (Amelx) and dentin sialofosfoprotein (DSPP) in teeth with Amelogenesis imperfecta (AI), dentinogenesis imperfecta (DI) and regional odontodysplasia (RO). Study Design In the present study we included patients who signed informed consent and agree to donate exfoliated deciduous teeth and third molars. This study analyzed six teeth with hypoplastic AI (HpAI), five teeth with hypocalcified AI (HcAI) three cases of Hipomature AI (HmAI), two teeth with DI and two teeth with RO and seven normal teeth. All cases were non-syndromic forms. Amelx C-19 and Amelx F-11 antibodies were used to detect amelogenin, and DSPP antibodies LFMb 21 and ab122321 were used for DSPP. For the characterization and diagnosis of each case anamnesis, clinical and radiographic examination was performed. Results AI cases: Only in cases of HcAI was sufficient enamel matrix left after decalcification, in these cases Amelx was detected in the interrods region. In dentine Amelx was not detected, DSPP was detected in peritubular dentin in most cases. DI cases When decalcifying these teeth, the enamel was lost. In dentin, DSPP was only detected with the Ab122321 antibody in the peritubular area. The antibody LFMb21 was negative in dentin. RO cases Temporary tooth enamel marked positive for Amlex and for DSPP. the dentin of the temporal tooth marked scarce marking for DSPP. In the permanent tooth the enamel was lost when decalcifying and in dentine no DSPP was detected. Conclusions The teeth with AI lost the enamel when decalcifying, except those with HcAI that presented Amelx in the enamel matrix. Dentin presented normal distribution of DSPP in AI teeth. The teeth with DI did not present DSPP in dentin. The teeth with OR presented anomalous marking of these proteins in enamel and dentin.