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Abstract
The location of specific proteins in sections through the lens of amphibians (Ranatemporaria,Xenopuslaevis), stained beforehand with azan and mounted in balsam, was studied by the indirect fluorescent antibodies method. Lenses from normal animals and lenses induced in vitro in the ectoderm of the amphibian gastrula were analyzed. To remove balsam, the lens sections were washed successively in xylol, ethanol, and physiological saline (pH 7.1). They were then treated in accordance with the general principles of immunohistochemical analysis. It is shown that specific antigens can be detected by this method in sections through the lens kept in balsam for 1 year. In sections kept for longer periods (2–3 years) the immunofluorescence test for lens proteins was negative.
Published Version
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