Abstract
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Highlights
The aim of the study was to test the applicability of commercially available poly- and monoclonal antibodies intended for human nervous tissue, for immunohistochemical detection of central nervous tissue from farm animals in meat products
Immunoreaction specific for nervous tissue was only demonstrated by monoclonal anti-glial fibrillary acidic protein (ICN Biomedicals) and polyclonal anti-myelin basic protein (DakoCytomation) antibodies
Good results were obtained with the demonstration of myelin basic protein because no marked weakening of immunoreaction was recorded in brain tissue after 30 min heat treatment with boiling
Summary
The aim of the study was to test the applicability of commercially available poly- and monoclonal antibodies intended for human nervous tissue, for immunohistochemical detection of central nervous tissue from farm animals in meat products. Monoclonal anti-neuron specific enolase antibody (anti-NSE, γγ enolase) was used for IHC detection of CNS tissue in heat-treated sausages containing different proportions (0, 7.4 and 33.3%) of bovine brain tissue. The. Aupperle et al (2002) tested the possibility of the use of the following primary antibodies in raw CNS tissue samples and in heat-treated meat products (80 or 120 °C for 20 min) containing 0, 0.5, 1 and 2% of brain tissue: anti-neuron specific enolase (antiNSE, M 873, DAKO, Hamburg, Germany), anti-glial fibrillary acidic protein (antiGFAP, Z 334, DAKO, Hamburg, Germany), anti-neurofilament (anti-NF, MAB 5274, Chemicon, Hofheim, Germany), anti-myelin basic protein (anti-MBP, 1118099, Boehringer, Mannheim, Germany) and anti-peripherin (NCL-PERIPH, Medac GmbH, Wedel, Germany). In case anti-NF antibody was used, the intensity of immunostaining after the heat treatment was lower, and anti-GFAP and anti-NSE antibodies showed to be unsuitable because of a very weak immunoreaction and a non-specific reaction of the background
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