Abstract

To characterize the immune cells present in different forms of feline anterior uveitis. Eyes were obtained from 49 cats diagnosed with chronic idiopathic lymphoplasmacytic anterior uveitis, 7 cats with feline infectious peritonitis (FIP), and 9 cats euthanized for nonocular disease. H&E sections were scored on the level of infiltrate in the anterior uvea. Immunohistochemistry was performed for FoxP3, CD3, and IL-17A, and positive cells were quantified in multiple images of each sample. A generalized estimating equationtested for an association between the level of inflammation and the prevalence of these cell types. Cells stained positive for IL-17A in idiopathic uveitis but not in FIP samples. We found significantly fewer FoxP3+ and CD3+ cells in low-grade compared with high-grade inflammation in idiopathic uveitis or FIP samples (P values all <.005), but no difference between FIP and high-grade samples. Idiopathic, but not FIP-associated, uveitis appears to have Th17 cell involvement. The numbers of FoxP3+ and CD3+ T-cells present appear directly correlated; thus, the severity of disease does not appear directly determined by the numbers of regulatory cells.

Highlights

  • Feline anterior uveitis is a clinically common condition, typically presenting bilaterally with no age or breed predispositions

  • Immunohistochemistry was performed for FoxP3, CD3 and IL-17A, and positive cells were quantified in multiple images of each sample

  • Cells stained positive for IL-17A in idiopathic uveitis but not in feline infectious peritonitis (FIP) samples

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Summary

Objective

To characterise the immune cells present in different forms of feline anterior uveitis. Eyes were obtained from 49 cats diagnosed with chronic idiopathic lympho-plasmacytic anterior uveitis, 7 cats with feline infectious peritonitis (FIP), and 9 cats euthanized for nonocular disease

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