Immunohistochemical biomarkers in patients with early-onset breast carcinoma by tissue microarray

Abstract

9589 Background: Young women with breast cancer have a poor prognosis and the role of biologic markers in young women is not well defined. The purpose of the current study is to investigate estrogen receptor (ER), progesterone receptor (PR), bcl-2, HER-2/neu, p53, and Ki-67 for their relation with clinicopathologic features and outcome. Methods: A cohort of early-onset breast cancer patients ( 105 patients) treated with conservative surgery and radiotherapy were entered in this study. Age range was 25–45 and median follow-up was 8.7 years. Each of the paraffin-embedded specimens was immunologically stained for 6 biomarkers by recently developed tissue microarray method. Results: The 10 year overall, breast relapse free and distant relapse free survival rates were 82.7%, 83.4%, and 68.1%, respectively, with 13 local relapse and 26 distant metastasis. The expression of ER, PR, bcl-2, HER-2/neu, p53 and Ki-67 were 42.7%, 48.5%, 35.6%, 28.0%, 36.9%, and 39.7%, respectively. PR negativity (p<0.05), Ki-67 posit...