Immunohistochemical assesment of heat shock protein 70 in adenoid tissue

Abstract

To evaluate the expression of heat shock protein 70 (HSP70) and its relation to histopathologic parameters in adenoid hypertrophy and hyperplasia. In addition, HSP70 expression in adenoid tissue was compared with in adult and childhood. Paraffin-embedded adenoid tissue sections were obtained from 19 childhood and 15 adult patients. Expression of HSP70 was evaluated by immunohistochemical staining using anti-HSP70 monoclonal antibody and correlated with histopathologic parameters. Positive HSP70 expression was observed mainly in the mucosal epithelium, lymphocytes in germinal centers, interfollicular lymphocytes, subepithelial plasma cells and vascular endothelium. HSP70 immunoreactivity in the mucosal epithelium with severe intraepithelial lymphocytic infiltration in childhood patients was higher than in adult patients. Although, the immunoreactivity of HSP70 was detected strongly in whole layer of metaplastic squamous epithelium, it was stained only in basal layers in respiratuary epithelium, Lymphocytes stained with HSP70 in germinal center and interfollicular areas of adenoid tissues was higher in childhood patients than in adults. These data suggest that HSP70 expression may have an important role in pathogenesis of adenoid hyperplasia, especially, in childhood.