Immunohistochemical and pathological changes in BALB/c mice immunized with whole sonicated Listeria monocytogenes antigens and the effect of probiotics

Thamer J Shihab,Zainab I Ibrahim

doi:10.33899/ijvs.2021.131562.1970

Abstract

The current study was undertaken to investigate the role of macrophages as a cellular immune function against immunization with whole sonicated Listeria monocytogenes antigens (WSLMAgs) and the effect of probiotics. A preparation of WSLMAgs containing whole L. monocytogenes cell, after two subcutaneous immunization of BALB/c mice with 0.5ml WSLMAgs (0.5mg/ml) at an interval of two weeks. The bacterial identification was conducted by a conventional culture method using Listeria selective media PALCAM and confirmed by Polymerase Chain Reaction (PCR), As well as, the immunohistochemical and pathological change of it was studied in vivo by inoculating mice with pre-challenge WSLMAgs and post-challenge with virulent L. monocytogenes (1×108 CFU/mL). The results revealed the cellular immune function against pre- and post-immunization in spleen organ via lymphocytic hyperplasia in white pulp and coalescence of lymphoid follicles and marker F4/80+ show the immune-positive cells in aggregation adjacent to lymphoid follicle or focal aggregation of macrophages between follicles. In conclusion, the effectiveness of sonicated L. monocytogenes pre and post-immunization then challenge with virulent L. monocytogenes in the induction of cellular immune response, might serve as an immunization platform for applicants.

Highlights

Listeriosis is a bacterial disease caused by Listeria monocytogenes that affects small ruminants [1]
The bacterial identification was conducted by a conventional culture method using Listeria selective media PALCAM and confirmed by Polymerase Chain Reaction (PCR), As well as, the immunohistochemical and pathological change of it was studied in vivo by inoculating mice with pre-challenge whole sonicated Listeria monocytogenes antigens (WSLMAgs) and post-challenge with virulent L. monocytogenes 1×108 CFU/mL
Listeria monocytogenes was utilized as a model organism in our investigations on the immunogenicity of lethally sonicated bacteria, as a result, Listeria monocytogenes serves as a rigorous model for evaluating lethal sonicated would retain bacterial immunogenicity and result in a superior vaccine when compared to other ways of pathogen death [5], In addition, the use of a sonicator in this study might have a significant influence on albino mice's cellular immunity [6]

Summary

Introduction

Listeriosis is a bacterial disease caused by Listeria monocytogenes that affects small ruminants [1]. Listeria monocytogenes, is a Gram-positive facultative rod-shaped bacterium, causes listeriosis, a serious and life-threatening illness caused mostly by ingesting contaminated foods [2], and is a food-borne intracellular pathogen that causes listeriosis is widespread in the world [3]. Listeria monocytogenes was utilized as a model organism in our investigations on the immunogenicity of lethally sonicated bacteria, as a result, Listeria monocytogenes serves as a rigorous model for evaluating lethal sonicated would retain bacterial immunogenicity and result in a superior vaccine when compared to other ways of pathogen death [5], In addition, the use of a sonicator in this study might have a significant influence on albino mice's cellular immunity [6]. CD8+ present in many local tissue macrophages, comprising the red pulp macrophages in the spleen, microglia in the brain, Kupffer cells in the liver, and Langerhans cells in the skin, are strongly and constitutively expressed [11]

Methods

Results

Conclusion