Immunohistochemical and morphometric study of pituitary pars distalis folliculostellate cells of nonpregnant and pregnant viscachas

Abstract

Pituitary folliculostellate cells (FSC) have several functions. Morphological, immunohistochemical and physiological characteristics of these cells have been reported for many mammalian species. There is little information, however, about the effect of pregnancy on FSC, particularly in wild animals. We conducted an immunohistochemical and morphometric study of the effect of pregnancy on FSC in viscacha, a seasonal breeder with a long gestation period. Immunohistochemistry and image analysis were used to measure the percentages of S-100-positive total, cellular, and colloidal areas and the number of FSC. In all groups studied, FSC were either isolated or arranged in small groups. These cells exhibited irregular nuclei, stellate shape, and cytoplasmic processes that delimited the follicles or contacted blood vessels. They expressed S-100 protein, which was localized both intracellularly (FSC) and extracellularly (follicular colloid). Some FSC were immunostained by anti-GFAP or anti-vimentin in the cytoplasm surrounding the nucleus and in the cytoplasmic processes, respectively. Morphometric parameters showed variation between nonpregnant and pregnant females. These variations were greatest during mid-pregnancy. We demonstrated that distribution of the FSC in pituitary pars distalis and expression of S-100 protein differ in nonpregnant and pregnant viscachas. Our findings suggest that FSC are involved in intrapituitary regulation of the hormone-secreting cells during pregnancy, probably by a paracrine mechanism.