Abstract
Background: Cholangiocellular carcinoma (CCC) is a very rare tumor entity that evolves from small bile ducts. Because treatment options are limited better knowledge of the tumor physiology is needed. For this purpose, basic research is dependent on cell lines. As there are only few CCC cell lines disposable, we aimed to isolate new CCC cell lines from surgical samples. Methods: The cell line was established from a 59-year-old Caucasian male patient who suffered from malignant pleural effusion of a distal cholangiocellular carcinoma (CCC). The cell line was established by explant culture and further characterized with immunohistochemistry and Western blotting. Mutational analysis was performed by means of AmpliSeq Cancer Panel v2. Additionally, we tested sensitivity towards commonly used chemotherapeutics by means of MTT assay. Results: Immunohistochemistry and Western blotting showed expression of tumor markers typical for CCC cell lines such as CK7, CK18-8, Sox9, STAT3 and ERK. Analysis of sensitivity towards chemotherapeutics revealed that Irinotecan was the only agent with significant effect on cell proliferation. Mutational analysis identified different mutations in important oncogenes and tumor suppressor genes such as EGFR, p53 and NOTCH1. Conclusion: The new cell line displays the typical characteristics of a CCC cell line. This new cell line might help to add new insights into this tumor entity and thereby help to find better treatment options for patients with CCC.
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