Abstract
BackgroundADAMTS expression can be associated with several inflammatory processes, and has been correlated with tumorigenesis of some neoplasms, but its participation in the development of periapical lesions has not been investigated. Therefore, our objective was to verify the expression of ADAMTS-1, versican and pEGFR in Periapical Granuloma (PG) and in the Radicular Cyst (RC) since they are the most common lesions of the periapex.Methods25 samples of RC and 10 of PG were used. As a control, 10 samples of inflammatory fibrous hyperplasia (IFH) and 10 of dental follicle (DF) were used. The expression of these proteins was investigated using immunohistochemistry.ResultsIn the epithelium of RC, IFH and DF, the expression of ADAMTS-1 was greater in DF than in RC (p < .001). Versicano showed greater expression in IFH than in RC, DF than in RC (p < .001). pEGFR showed greater expression in IFH and RC than in DF (p < .01 and p < .05, respectively). In connective tissue, ADAMTS-1 expression was greater in PG and RC than in IFH and DF (p < .001). Versicano showed greater expression in PG, RC and IFH compared to DF (p < .001). In pEGFR there was a higher expression in PG when compared to RC, IFH and DF (p < .001). Greater immunostaining occurred in the RC than in the DF (p < .001).ConclusionsOur results suggest that the studied proteins may participate in the pathogenesis of PG and RC, through the interaction of these proteins, in the remodeling of the ECM (versican) by ADAMTS-1, producing bioactive fragments, which could activate EGFR, contributing to the formation, growth and maintenance of injuries.
Highlights
ADAMTS expression can be associated with several inflammatory processes, and has been correlated with tumorigenesis of some neoplasms, but its participation in the development of periapical lesions has not been investigated
Considering that the interaction mechanism among ADAMTS-1, versican and phosphorylated epidermal growth factor receptor can influence the etiopathogenesis of periapical lesions, this study aimed to investigate the immunohistochemical expression of ADAMTS-1, versican and pEGFR in Radicular Cyst (RC) and Periapical Granuloma (PG)
The ADAMTS-1 expression was significantly higher in PG and RC when compared to inflammatory fibrous hyperplasia (IFH) and dental follicle (DF) (p < .001)
Summary
ADAMTS expression can be associated with several inflammatory processes, and has been correlated with tumorigenesis of some neoplasms, but its participation in the development of periapical lesions has not been investigated. Our objective was to verify the expression of ADAMTS-1, versican and pEGFR in Periapical Granuloma (PG) and in the Radicular Cyst (RC) since they are the most common lesions of the periapex. Periapical granuloma (PG) consists of a chronically inflamed granulation tissue, which is the most common periapical lesion, representing about 70 % of all lesions in the periapex [1]. The proteolysis of the extracellular matrix (ECM) has been widely related to ADAMTS (A Disintegrin and Metalloproteinase with Thrombo Spondin Motifs) endopeptidases [6]. It is suggested that dental pulp cells (odontoblasts, cementoblasts, cementocytes, osteoblasts, osteocytes and periodontal ligament cells) regulate the expression of ADAMTS-1, ADAMTS-4, ADAMTS-5 and versican substrate [8]
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