Abstract
Aim of the study: Immunohistochemical evaluation of hormone receptors, Her2/neu, CK5/6, E-cadherin, beta-catenin, p53 and PTEN on Tissue Micro Array (TMA) of 46 Moroccan invasive breast carcinomas. Materials and Methods: The cases comprised 40 invasive ductal carcinomas, 4 invasive lobular carcinomas, 1 mixed carcinoma and 1 invasive colloid carcinoma. TMA paraffin blocs were prepared with the Beecher manual arrayer and immunostaining was performed using standard immunoperoxidase techniques. Results: 58.69% of the cases were ER positive. 43.18% (19/44) were triple negative breast cancers (TNBC) of which 15.78% (3/19) were of the basal phenotype expressing CK5/6. On the other hand, 72.22% (13/18) of the TNBC cases were IDC grade 3. Of the 18 IDC grade 3, 22.22% (4/18) were CK5/6 positive. 41.30% and 10.86% of the cases showed reduced expression of E-cadherin and beta-catenin respectively. Beta-catenin nuclear and cytoplasmic staining was noted in 20% and 97.82% respectively. p53 was overexpressed in 10.86% of the cases whereas PTEN loss or reduced expression was noted in 86.95% of the cases. Conclusion: The aim of our study was to introduce TMA technique in our hospital which is considered a reference institution for cancer in Morocco. Although no statistical study was performed to look for any significance of the results obtained, we found good correlation with some of the data in the literature. To determine the molecular characteristics, if any, of the Moroccan patient, larger multidisciplinary and prospective studies would be interesting in the aim to personalize therapeutic decisions.
Highlights
Tissue Microarray (TMA) allows analysis of hundreds of specimens with one slide instead of incubating and analyzing samples one slide at a time
Beta-catenin nuclear and cytoplasmic staining was noted in 20% and 97.82% respectively. p53 was overexpressed in 10.86% of the cases whereas PTEN loss or reduced expression was noted in 86.95% of the cases
Among the multiple markers reported in the literature, we chose to study the protein expression of oestrogen (ER) and progesterone (PR) hormone receptors, Her2/neu, 2 suppressor genes PTEN and p53, 2 proteins of the Wnt signalling pathway E-cadherin and beta-catenin and CK5/6
Summary
Tissue Microarray (TMA) allows analysis of hundreds of specimens with one slide instead of incubating and analyzing samples one slide at a time. All the histochemical and molecular detection techniques that can be used with regular sections can be used with tissue microarrays. Typical applications include immunohistochemical detection of protein expression of tumor specimens. It is a tool in routine practice for quality control of immunohistochemistry techniques especially for hormone receptors and Her2/neu. To introduce this technique in our institution, we chose to apply it to a set of Moroccan invasive breast carcinomas and study the immunohistochemical expression of 8 biomarkers of diagnostic, therapeutic and prognostic interest. Among the multiple markers reported in the literature, we chose to study the protein expression of oestrogen (ER) and progesterone (PR) hormone receptors, Her2/neu, 2 suppressor genes PTEN and p53, 2 proteins of the Wnt signalling pathway E-cadherin and beta-catenin and CK5/6
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