Abstract
The overall success of nanocarriers in biomedical applications depends on their interaction with different proteins in blood. Immunoglobulins as a major protein class of the blood proteome may considerably influence the identity of the nanocarriers in blood. However, there is a lack of knowledge about the specific details of the interaction mechanism between different immunoglobulins and nanocarriers. Therefore, the authors have investigated the interaction of different immunoglobulin classes-namely, immunoglobulin G, A, and M-with different polystyrene model nanoparticles. The authors report that immunoglobulin interaction with nanoparticles strongly depends on the immunoglobulin class and surface charge of the nanoparticles. Furthermore, upon adsorption on the nanoparticles' surfaces, aggregation processes and denaturation of immunoglobulins were observed. This highlights the importance of nanocarriers' design in order to prevent unfavorable denaturation and adsorption processes of immunoglobulins on nanoparticle surfaces.
Highlights
The desired overall success of nanocarriers (NCs) in medicine strongly depends on the interactions that NCs undergo in the organism
We investigated the influence of different immunoglobulins in the protein corona of differently charged polystyrene nanoparticles
The net charge of nanoparticles was influenced by adsorption of Igs, and aggregation processes were induced in some cases
Summary
The desired overall success of nanocarriers (NCs) in medicine strongly depends on the interactions that NCs undergo in the organism. The role of the different Igs in the protein corona of nanocarriers is of importance Their interaction mechanism has to be fully understood in order to minimize NC-Ig interactions as much as possible, resulting in a better chance that NCs will not be cleared by the immune system or even induce adverse effects. In order to draw general conclusions for interaction trends between Igs and differently charged NCs, model NCs with different physicochemical properties are needed For this we used polystyrene nanoparticles (PS-NPs) with different functional groups (unfunctionalized, carboxy-functionalized, and amino-functionalized) as model systems for the investigation of interactions with IgG, IgA, and IgM from human plasma. The structural stability of immunoglobulins after adsorption was analyzed via nanodifferential scanning fluorimetry (nanoDSF)
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