Abstract
Summary Macaca mulatta monkeys immunized to bovine serum albumin and the dinitrophenyl antigenic determinant demonstrated immediate type skin reactivity and a 24-hr reaction of cutaneous necrosis. The serum of three of the four monkeys immunized transferred immediate type skin reactivity. Precipitating antibody, migrating in the γ-globulin region was synthesized in response to both DNP and BSA determinants. Anti-BSA antibody of the IgA and two specificities of IgG types were demonstrated by radioimmunoelectrophoresis. Anti-BSA IgM was detected in purified and concentrated pooled hyperimmune anti-BSA serum. Anti-DNP antibody of the IgM and IgG types was detected. The precipitating capacity of the sera was measured by the direct radioactive antigen precipitin technique; and the formation of soluble antigen-antibody complexes in antibody excess was found in the DNP-anti-DNP system. The formation of soluble complexes was shown to be more marked when DNP-BGG of a lower degree of haptenization was reacted with the anti-DNP serum. Equilibrium and dilution factors were measured and found to contribute little to the formation of soluble complexes. Classical quantitative precipitin types of curves were constructed. Anti-BSA precipitated in combination with antigen around a 3:1 ratio on a molar basis, with a sharp zone of equivalence. Anti-DNP combined with a broader zone of equivalence, than was observed in the BSA-anti-BSA system.
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