Abstract
Enhancer RNAs, a type of long non-coding RNAs (lncRNAs), play a critical role in the occurrence and development of glioma. RNA-seq data from 161 glioblastoma multiforme (GBM) samples were acquired from The Cancer Genome Atlas database. Then, 70 eRNAs were identified as prognosis-related genes, which had significant relations with overall survival (log-rank test, p < 0.05). AC003092.1 was demonstrated as an immune-related eRNA by functional enrichment analysis. We divided samples into two groups based on AC003092.1 expression: AC003092.1 High (AC003092.1_H) and AC003092.1 Low (AC003092.1_L) and systematically analyzed the influence of AC003092.1 on the immune microenvironment by single-sample gene-set enrichment analysis and CIBERSORTx. We quantified AC003092.1 and TFPI2 levels in 11 high-grade gliomas, 5 low-grade gliomas, and 7 GBM cell lines. Our study indicates that AC003092.1 is related to glioma-immunosuppressive microenvironment, and these results offer innovative sights into GBM immune therapy.
Highlights
In the human central nervous system, gliomas are the most common primary tumor
There are 2,695 Long non-coding RNAs (lncRNAs) transcripts, and 2,303 predicted target genes have been identified by the PreSTIGE algorithm (Vucicevic et al, 2015)
The Enhancer RNAs (eRNAs)-target genes pairs were confirmed by this eRNA transcripts database
Summary
In the human central nervous system, gliomas are the most common primary tumor. Glioblastoma multiforme (GBM) is the most malignant and has the worst prognosis. Radiotherapy, and chemotherapy were applied, the treatment effect of patients with glioblastoma is still worse (Cuddapah et al, 2014). The median survival time for patients with glioblastoma is only 14 months (Weller et al, 2015). Long non-coding RNAs (lncRNAs) are a type of non-coding RNAs longer than 200 nucleotides (Wapinski and Chang, 2011; Spizzo et al, 2012).
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