Abstract
The present study deals with the immunogenicity of sulfatide. Despite its acidic character, we succeeded in raising high titer antibodies. The most effective immunization procedure involved repeated intravenous injections of a mixture of sulfatide-lecithin-cholesterol-methylated bovibe serum albumin (1:4:10:10). The respective role of the protein carrier and of the auxiliary lipids in inducing the antigencity was studied. The antibodies against sulfatide were purified by an immunoadsorption method and were characterized. They fixed complement and agglutinated. Nitrocatechol sulfate could inhibit the antibody reaction completely at high concentrations, while galactose and methyl galactoside had only a weak effect.
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