Abstract
Immunogenicity of Multiple Antigenic Peptides (MAP) Based on B and T cell Epitopes of E2 Glycoprotein of Chikungunya Virus in Murine System
Highlights
Chikungunya is a febrile illness caused by positive sense RNA virus that belongs to genus Alphavirus of the Togaviridae family
Multiple Antigenic Peptide (MAP) appears to be an alternate approach for vaccine design
In this study different formulation of MAPs were used, MAP entrapped in microspheres with CpG ODN showed highest peak antibody titers and sustained over longer duration with strong memory response after boosting with native protein, followed by MAP + murabutide formulation as compared to without adjuvant
Summary
Chikungunya is a febrile illness caused by positive sense RNA virus that belongs to genus Alphavirus of the Togaviridae family. We focused our attention on E2 glycoprotein because of its involvement in host-cell receptor interaction with immune cells [11] It can be a good target for virus neutralization/ blocking antibody for receptor on the antigen presenting cells by the virus. Previous studies showed that monoclonal antibody against the specific epitope of E2 protein protected mice or inhibited virus entry inside the vero cells after CHIKV challenge [1215]. These observations prompted us to use B and T cell epitopes of E2 protein in developing MAP based immunogen. Previous studies showed improved immune response can be achieved for different disease using MAP approach [19,20,21]. MAP entrapped in PLGA microspheres with CpG ODN or Murabutide adjuvant
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