Abstract
The SAL rat leukaemia, which resembles acute myeloblastic leukaemia, appeared initially to be non-immunogenic since resistance to an i.p. challenge with as few as 100 cells could not be obtained using stimulation of the RES or by immunization with SAL cells exposed to x-rays, nitrogen mustard, iodoacetate or glutaraldehyde. However, immunization with SAL cells exposed to low doses of mitomycin-C slowed the growth of the challenge inoculum. Cells treated with high doses of mitomycin-C did not immunize. The results are interpreted in terms of rapid shedding of a tumour-specific antigen from the membrane of SAL cells.
Highlights
Summary.-The SAL rat leukaemia, which resembles acute myeloblastic leukaemia, appeared initially to be non-immunogenic since resistance to an i.p. challenge with as few as 100 cells could not be obtained using stimulation of the RES or by immunization with SAL cells exposed to x-rays, nitrogen mustard, iodoacetate or glutaraldehyde
For this nitrogen mustard (Mustine hydrochloride, study we used a cross-linking agent; glutaraldehyde, a sulphydryl blocking agent; sodium iodoacetate, and 2 antimitotic drugs: nitrogen mustard (Mustine) and mitomycin-C. Both glutaraldehyde and sodium iodoacetate have been shown to improve the immunogenicity of tumour cells in other
Cells killed by treatment with iodo- procedures such as exposure to x-rays, acetate (Apffel et at., 1966; Wang and which are used to sterilize cells for Halliday, 1966), nitrogen mustard (Ishi- immunization, is likely to reduce to a date et al, 1965) or glutaraldehyde greater extent the concentration of TSTAs (Sanderson and Frost, 1974) have all been in the membrane of tumours with the found to immunize syngeneic mice against higher rate of shedding
Summary
Summary.-The SAL rat leukaemia, which resembles acute myeloblastic leukaemia, appeared initially to be non-immunogenic since resistance to an i.p. challenge with as few as 100 cells could not be obtained using stimulation of the RES or by immunization with SAL cells exposed to x-rays, nitrogen mustard, iodoacetate or glutaraldehyde. Preparation of cells for immunization.SAL spleen cells were washed and resuspended in TC 199 at a concentration of 5 x 107 cells/ml and immediately x-irradiated at a dose rate of 800 rad/min from a 200 kV Marconi x-ray machine (no filter) at room temperature and under well oxygenated conditions.
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