Year-end Sale % OFF 
Abstract
BackgroundTuberculosis (TB) is a major global public health problem. New treatment methods on TB are urgently demanded. In this study, Mycobacterium tuberculosis (MTB) rv2190c DNA vaccine was prepared and its immunogenicity and therapeutic effects were evaluated.ResultsNon-infected mice immunized with rv2190c DNA or ag85a DNA showed higher levels of interferon-gamma (IFN-γ) in stimulated spleen lymphocyte culture supernatants, and had more Th1 cells and an elevatory ratio of Th1/Th2 immune cells in whole blood, indicating that Th1-type immune response was predominant. Compared with the saline group, ag85a DNA group and rv2190c DNA group in the infected mice decreased the lung colony-forming units (CFUs) by 0.533 and 0.283 log10, and spleen CFUs by 0.425 and 0.321 log10 respectively, and pathological lesion.ConclusionsThe rv2190c DNA had some immunotherapeutic effect on TB.
Highlights
Tuberculosis (TB) is a major global public health problem
It is difficult to cure with anti-TB chemotherapy because a long treatment duration with multiple drugs was used on active TB, many TB patients cannot complete a full period of treatment, which lead to failure of treatment and acquired drug resistance with eventual creation of multi-drugresistant TB (MDR-TB) [4]
We report that the rv2190c gene of Mycobacterium tuberculosis (MTB) is effective as a therapeutic DNA vaccine
Summary
Tuberculosis (TB) is a major global public health problem. New treatment methods on TB are urgently demanded. Mycobacterium tuberculosis (MTB) rv2190c DNA vaccine was prepared and its immunogenicity and therapeutic effects were evaluated. Immunotherapy with plasmid DNA has been found to be an effective adjunctive treatment in combination with antibacterial chemotherapy in mice. It both shortened the period of treatment and improved the therapy outcome of latent TB infection [8, 9]. We and others have shown that ag85a DNA vaccine can have immunotherapeutic effects against MDR-TB in mice [10,11,12]. We report that the rv2190c gene of Mycobacterium tuberculosis (MTB) is effective as a therapeutic DNA vaccine. Målen et al [14] identified the protein product of rv2190c in MTB
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
